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(>)Tj /F5 1 Tf .7714 0 TD [(1000\))-997.5(\(320Ð)]TJ /F12 1 Tf 5.8108 0 TD (>)Tj /F5 1 Tf .7714 0 TD [(1000\))-22451.2(\(1.3Ð3.7\)/\(0.9Ð1.1\))]TJ 0 7.9702 -7.9702 -0 421.8519 65.3102 Tm [(Pos)6.4(itive)-333.8(samp)8.2(les)-335.9(in)-327.8(bold.)]TJ 0 5.9774 -5.3127 -0 427.2944 65.3102 Tm (a)Tj 0 7.9702 -7.9702 -0 430.8093 68.2583 Tm [(Perform)5.3(ed)-336.4(for)-331.1(99)-330.2(samp)8.2(les.)]TJ 0 5.9774 -5.3127 -0 436.2519 65.3102 Tm (b)Tj 0 7.9702 -7.9702 -0 439.7668 68.5984 Tm [(Titer:)-329(1:10)7.3(0.)]TJ -.4126 -1.131 TD [(VNT,)-330.3(v)7.2(irus)-330.9(neutral)5.4(ization)-332(test)5.8(.)]TJ /F3 1 Tf 8.9663 0 0 8.9663 299.6786 34.9228 Tm (784)Tj ET endstream endobj 69 0 obj <>/XObject<>/ProcSet [/PDF /Text /ImageB /ImageC /ImageI]/Font<>>>/Thumb 71 0 R/MediaBox[0 0 612 792]/Rotate 0>> endobj 72 0 obj <>/ProcSet[/PDF/Text]/Font<>>> endobj 70 0 obj <>stream /GS1 gs BT /F5 1 Tf 8.9663 0 0 8.9663 59.7543 303.8173 Tm 0 0 0 1 k 0 Tc 0 Tw [(TOSV)-253.9(immunoglobulins.)-258.7(In)-252.1(addition,)-256.1(a)-252.4(signiÞcant)-255.8(number)-253.9(of)]TJ 0 -1.2203 TD [(the)-364.2(samples)-358.6(\(99/120;)-358(82.5%\))-357.6(were)-356.2(investigated)-367.9(for)-358(the)-357.8(pres-)]TJ T* [(ence)-280.7(of)-281.4(neutralizing)-282.5(antibodies)-281.1(to)-275.1(SFSV,)-279(SFNV,)-280.5(and)-277.8(TOSV)-279.2(as)]TJ 0 -1.2267 TD [(well.)-264.4(Since)-263.8(IgM)-258.2(for)-263.2(TOSV)-260.3(and)-258.8(other)-266.5(SFVs)-259.9(were)-261.4(only)-260.7(detected)]TJ 0 -1.2203 TD [(in)-361(a)-359.9(small)-359.5(number)-361.4(of)-363.6(samples)-358.6(in)-361(the)-364.2(study)-358.3(group)-364.7(via)-357.9(IIFT/)]TJ T* [(ELISA)-286.2(\(n:8\))-285(and)-284.1(VNTs)-279(were)-286.7(observed)-284.6(to)-281.4(be)-282.5(negative,)-286.7(we)-279.7(did)]TJ T* [(not)-386(perform)-382.8(the)-389.5(IB)-378.2(assay)-385.4(for)-383.3(IgM)-378.4(reactive)-389.7(samples)-383.9(or)-382.8(com-)]TJ 0 -1.2267 TD [(pared)-257.4(IgM)-251.9(results.)-258.1(This)-251.5(constitutes)-260.9(the)-256.7(main)-256.9(limitation)-252.3(of)-256.2(this)]TJ 0 -1.2203 TD [(study.)-190.5(Nevertheless,)-191(viral)-185.1(RNA)-189(detection)-189.8(has)-187.6(been)-184.6(established)]TJ T* [(as)-272.7(a)-265.1(powerful)-271(tool)-275.1(and)-265.1(an)-268.5(effective)-276.5(alternative)-270.9(for)-269.5(identifying)]TJ 0 -1.2266 TD [(acute)-240.8(TOSV)-235(infections)-239.3(\(Charel)-235.3(et)-234.3(al.)-233.6(2005\).)-235.4(The)-233.9(impact)-235.7(of)-237.2(viral)]TJ 0 -1.2203 TD [(RNA)-429.2(detection)-423.7(in)-424.2(TOSV-associated)-429.4(central)-424.1(nervous)-427.5(system)]TJ 28.0042 13.4425 TD [(infections)-334.2(in)-335.7(the)-332.5(study)-333(region)-337.2(has)-333(also)-329.6(been)-336.4(documented)-333.1(re-)]TJ 0 -1.2203 TD [(cently,)-418.6(where)-422.8(TOSV)-418.3(IgM)-416.3(was)-419.8(identiÞed)-424.4(via)-414.8(IIFT)-422.6(in)-417.9(only)-418.8(4)]TJ T* [(\(25%\))-216.6(of)-211.9(the)-218.7(16)-214(TOSV)-216(RNA)-214.3(positive)-222.3(sera)-216.4(\(Ergu)]TJ 19.5315 .0063 TD (¬)Tj .4742 -.0063 TD [(nay)-209.7(et)-215.3(al.)-214.6(2010\).)]TJ -18.8929 -1.2267 TD [(For)-444.2(TOSV)-443.6(IgG,)-444.5(the)-452.7(commercial)-446.3(assays)-443.7(evaluated)-450.5(in)-443.2(this)]TJ -1.1128 -1.2203 TD [(study)-282.4(were)-280.3(observed)-284.6(to)-281.5(display)-282.2(slight)-282.2(to)-281.4(fair)-282.4(agreement)-284(with)]TJ T* [(an)-179.9(average)-181.7(percentage)-185.9(of)-180.3(57.8%.)-183(This)-181.9(relatively)-180.6(low)-185(agreement)]TJ T* [(observed)-316.2(is)-314.7(presumably)-323.9(due)-314.1(to)-319.4(the)-313.6(viral)-317.8(antigens)-319.4(employed,)]TJ 0 -1.2267 TD [(as)-399.2(well)-400.6(as)-399.2(differences)-403.9(in)-398.9(assay)-398.1(conÞgurations,)-408.2(as)-399.2(displayed)]TJ 0 -1.2203 TD [(previously)-184(\(Calisher)-183.2(et)-183.7(al.)-183(1999,)-178(Cusi)-183.4(et)-183.7(al.)-176.7(2001,)-184.3(Di)-182(Bonito)-180.3(et)-183.7(al.)]TJ T* [(2002\).)-229.1(While)-237(virus-infected)-239.4(cells)-235.2(are)-234.1(the)-231.4(source)-232.9(of)-237.2(the)-231.4(antigens)]TJ 0 -1.2266 TD [(in)-398.9(the)-402.1(IIFT,)-400.3(recombinant)-400.6(N)-398.3(protein)-404.1(of)-401.6(TOSV)-399.4(is)-403.2(used)-397.6(in)-398.9(the)]TJ 0 -1.2203 TD [(ELISA)-267.2(and)-265.1(IB,)-267.3(although)-271.8(in)-266.2(different)-277.4(matrices.)-264.4(The)-271.9(TOSV)-266.6(IIFT)]TJ ET 59.811 720.057 490.11 .22675 re f 59.811 365.442 490.11 .22675 re f 124.611 700.781 172.913 .22681 re f 432.567 700.781 117.354 .22681 re f 59.811 683.093 490.11 .22675 re f BT /F7 1 Tf 8.9663 0 0 8.9663 138.0472 725.7258 Tm (Table)Tj /F5 1 Tf 3.0603 0 TD (3.)Tj /F7 1 Tf 1.2519 0 TD [(Assay)-334.5(Results)-334.7(for)-334.5(the)-332(Virus)-339.4(Neutralization)-331.8(Test)-335.4(Reactive)-335.5(Samples)]TJ /F8 1 Tf .3351 -2.0423 TD [(IIFT)-337.2(\(titer:)-336.4([1/x]\))-24122.2(VNT)-329.7(\(titer:)-336.4([1/x]\))]TJ -13.373 -2.0044 TD [(Sample)-336(no.)-2934.1(SFSV)-3025.8(SFNV)-2883.7(TOSV)-2945.5(SFCV)-2873.8(ELISA)-337.6(\(ratio\))-2911.1(IB)-2905.7(SFSV)-2741.2(SFNV)-2738.2(TOSV)]TJ /F5 1 Tf 0 -2.0992 TD [(1)-7821(n)-5106(320)-3836.5(n)-4935.3(n)-5504.3(0.459)-4401.7(1)]TJ /F11 1 Tf 38.0702 0 TD (þ)Tj /F5 1 Tf 4.4071 0 TD [(n)-4182.8(160)-4317.1(n)]TJ -42.4773 -1.1065 TD [(2)-7821(n)-5106(320)-3836.5(n)-4935.3(n)-5504.3(0.391)-4401.7(1)]TJ /F11 1 Tf 38.0702 0 TD (þ)Tj /F5 1 Tf 4.4071 0 TD [(n)-4182.8(160)-4317.1(n)]TJ -42.4773 -1.1128 TD [(3)-7821(n)-5106(320)-3836.5(n)-4935.3(n)-5504.3(0.496)-4401.7(2)]TJ /F11 1 Tf 38.0702 0 TD (þ)Tj /F5 1 Tf 4.4071 0 TD 4.6823 Tc [(n8)4682.3(0)364.7(n)]TJ -42.4773 -1.1128 TD 0 Tc [(4)-7498.5(1000)-3829.7(n)-4935.3(n)-4935.3(n)]TJ /F6 1 Tf 30.919 0 TD (1.305)Tj /F5 1 Tf 6.9995 0 TD [(n)-3525.2(320)-4178(n)-4783.5(n)]TJ -37.9185 -1.1128 TD [(5)-7998(100)-3830.2(n)-4935.3(n)-4935.3(n)-5504.3(0.245)-4749.5(n)-3525.2(320)-4178(n)-4783.5(n)]TJ 0 -1.1065 TD [(6)-7998(320)-3830.2(n)-4935.3(n)-4935.3(n)-5504.3(0.481)-4401.7(1)]TJ /F11 1 Tf 38.0702 0 TD (þ)Tj /F5 1 Tf 3.9455 0 TD [(320)-4178(n)-4783.5(n)]TJ -42.0157 -1.1128 TD [(7)-7998(100)-3830.2(n)-4935.3(n)-4935.3(n)-5504.3(0.718)-4401.7(1)]TJ /F11 1 Tf 38.0702 0 TD (þ)Tj /F5 1 Tf 3.9455 0 TD [(320)-4215.9(80)-4317.6(n)]TJ -42.0157 -1.1128 TD [(8)-7998(320)-3830.2(n)-4935.3(n)-4935.3(n)-5504.3(0.162)-4401.7(1)]TJ /F11 1 Tf 38.0702 0 TD (þ)Tj /F5 1 Tf 3.9455 0 TD [(320)-4178(n)-4783.5(n)]TJ -42.0157 -1.1128 TD [(9)-7998(320)-3830.2(n)-4935.3(n)-4935.3(n)-5504.3(0.289)-4749.5(n)-3525.2(320)-4178(n)-4783.5(n)]TJ 0 -1.1065 TD [(10)-7498(100)-3830.2(n)-4935.3(n)-4935.3(n)-5504.3(0.093)-4749.5(n)-3525.2(320)-4178(n)-4783.5(n)]TJ 0 -1.1128 TD [(11)-7498(320)-3830.2(n)-4935.3(n)-4935.3(n)-5504.3(0.518)-4749.5(n)-4024.8(4)0(0)-4178.5(n)-4783.5(n)]TJ T* [(12)-7498(320)-3830.2(n)-4935.3(n)-4935.3(n)-5504.3(0.185)-4749.5(n)-4024.8(8)0(0)-4178.5(n)-4783.5(n)]TJ 0 -1.1065 TD [(13)-6998.5(1000)-3829.7(n)-4935.3(n)-4935.3(n)-5504.3(0.082)-4749.5(n)-3525.2(320)-4178(n)-4783.5(n)]TJ 0 -1.1128 TD [(14)-7321(n)-4935.3(n)-4606.5(1000)-3836.1(n)-5504.3(0.450)-4401.7(2)]TJ /F11 1 Tf 38.0702 0 TD (þ)Tj /F5 1 Tf 4.4071 0 TD 4.6444 Tc [(nn)259.2(4)4644.4(0)]TJ 6.7246 0 0 5.9768 540.8503 545.1022 Tm 0 Tc (a)Tj 8.9663 0 0 8.9663 59.811 531.1558 Tm [(15)-7321(n)-4935.3(n)-5106(320)-3836.5(n)-5504.3(0.358)-4401.7(2)]TJ /F11 1 Tf 38.0702 0 TD (þ)Tj /F5 1 Tf 4.4071 0 TD 4.6444 Tc [(nn)259.2(4)4644.4(0)]TJ 6.7246 0 0 5.9768 540.8503 535.1243 Tm 0 Tc (a)Tj 8.9663 0 0 8.9663 59.811 521.1778 Tm [(16)-7321(n)-4935.3(n)-4606.5(1000)-3836.1(n)-5504.3(0.507)-4401.7(2)]TJ /F11 1 Tf 38.0702 0 TD (þ)Tj /F5 1 Tf 4.4071 0 TD 4.6444 Tc [(nn)259.2(4)4644.4(0)]TJ 6.7246 0 0 5.9768 540.8503 525.203 Tm 0 Tc (a)Tj 8.9663 0 0 8.9663 59.811 511.2566 Tm [(17)-7321(n)-4935.3(n)-5106(320)-3836.5(n)]TJ /F6 1 Tf 30.919 0 TD (0.879)Tj /F5 1 Tf 6.6517 0 TD (2)Tj /F11 1 Tf .4995 0 TD (þ)Tj /F5 1 Tf 4.4071 0 TD 4.6444 Tc [(nn)259.2(4)4644.4(0)]TJ 6.7246 0 0 5.9768 540.8503 515.225 Tm 0 Tc (a)Tj 8.9663 0 0 8.9663 59.811 501.2786 Tm [(18)-7321(n)-4935.3(n)-4606.5(1000)-3836.1(n)]TJ /F6 1 Tf 30.919 0 TD (0.873)Tj /F5 1 Tf 6.6517 0 TD (2)Tj /F11 1 Tf .4995 0 TD (þ)Tj /F5 1 Tf 4.4071 0 TD 4.6444 Tc [(nn)240.3(4)4644.4(0)]TJ -42.4773 -1.1128 TD 0 Tc [(19)-7321(n)-4935.3(n)-4606.5(1000)-3836.1(n)-5504.3(0.684)-4401.7(1)]TJ /F11 1 Tf 38.0702 0 TD (þ)Tj /F5 1 Tf 4.4071 0 TD 4.6444 Tc [(nn)240.3(4)4644.4(0)]TJ -42.4773 -1.1128 TD 0 Tc [(20)-7321(n)-4935.3(n)-5106(320)-3836.5(n)-5504.3(0.724)-4401.7(2)]TJ /F11 1 Tf 38.0702 0 TD (þ)Tj /F5 1 Tf 4.4071 0 TD 4.6444 Tc [(nn)240.3(4)4644.4(0)]TJ -42.4773 -1.1065 TD 0 Tc [(21)-7321(n)-4935.3(n)-5106(320)-3836.5(n)-5504.3(0.366)-4401.7(2)]TJ /F11 1 Tf 38.0702 0 TD (þ)Tj /F5 1 Tf 4.4071 0 TD 4.6444 Tc [(nn)240.3(4)4644.4(0)]TJ -42.4773 -1.1128 TD 0 Tc [(22)-7321(n)-4935.3(n)-4606.5(1000)-3836.1(n)-5504.3(0.784)-4401.7(3)]TJ /F11 1 Tf 38.0702 0 TD (þ)Tj /F5 1 Tf 4.4071 0 TD 4.6444 Tc [(nn)240.3(4)4644.4(0)]TJ -42.4773 -1.1128 TD 0 Tc [(23)-7321(n)-4935.3(n)-4606.5(1000)-3836.1(n)-5504.3(0.633)-4401.7(2)]TJ /F11 1 Tf 38.0702 0 TD (þ)Tj /F5 1 Tf 4.4071 0 TD 4.6444 Tc [(nn)240.3(4)4644.4(0)]TJ -42.4773 -1.1065 TD 0 Tc [(24)-7321(n)-4935.3(n)-5106(320)-3836.5(n)-5504.3(0.157)-4749.5(n)-3986.8(n)-4644.4(n)-4404.1(4)0(0)]TJ 0 -1.1128 TD [(25)-7321(n)]TJ /F12 1 Tf 12.7343 0 TD (>)Tj /F5 1 Tf .7651 0 TD (1000)Tj /F12 1 Tf 4.7359 0 TD (>)Tj /F5 1 Tf .7714 0 TD [(1000)-3836.1(n)]TJ /F6 1 Tf 11.9124 0 TD (3.661)Tj /F5 1 Tf 6.6517 0 TD (3)Tj /F11 1 Tf .4995 0 TD (þ)Tj /F5 1 Tf 4.4071 0 TD 4.6444 Tc [(nn)240.3(4)4644.4(0)]TJ -42.4773 -1.1128 TD 0 Tc [(26)-7321(n)]TJ /F12 1 Tf 12.7343 0 TD (>)Tj /F5 1 Tf .7651 0 TD [(1000)-3507.3(1000)-3836.1(n)]TJ /F6 1 Tf 17.4196 0 TD (2.951)Tj /F5 1 Tf 6.6517 0 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